| Solution Information | help | |
| Enzyme: | cAMP-dependent protein kinase catalytic subunit alpha | |
| inhibitor: | BDBM14029 | |
| substrate: | Kemptide | |
| Solution Type: | Aqueous | |
| pH at Preparation: | 7.6 | |
| Temp. Prep.: | 30 | |
| Comments: | n/a | |
| Solute 1: | lactate dehydrogenase | |
| Concentration: | 10 ug/mL | |
| Concentration: | n/a | |
| Purity: | n/a | |
| Purification Meth.: | n/a | |
| Comments: | n/a | |
| Solute 2: | MgCl2 | |
| Concentration: | 10 mM | |
| Concentration: | n/a | |
| Purity: | n/a | |
| Purification Meth.: | n/a | |
| Comments: | n/a | |
| Solute 3: | ATP | |
| Concentration: | 10 -15 uM | |
| Concentration: | n/a | |
| Purity: | n/a | |
| Purification Meth.: | n/a | |
| Comments: | n/a | |
| Solute 4: | phosphoenolpyruvate | |
| Concentration: | 2.5 mM | |
| Concentration: | n/a | |
| Purity: | n/a | |
| Purification Meth.: | n/a | |
| Comments: | n/a | |
| Solute 5: | pyruvate kinase | |
| Concentration: | 50 ug/mL | |
| Concentration: | n/a | |
| Purity: | n/a | |
| Purification Meth.: | n/a | |
| Comments: | n/a | |
| Solvent 1: | water | |
| Concentration: | 1.0 | |
| Purity: | n/a | |
| Purification Meth.: | n/a | |
| Comments: | n/a | |
| Solvent 2: | DMSO | |
| Concentration: | 2.5% | |
| Purity: | n/a | |
| Purification Meth.: | n/a | |
| Comments: | n/a | |
| pH Buffer 1: | HEPES | |
| Concentration: | 100 mM | |
| ΔHion: | n/a | |
| Comments: | n/a | |
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